2014 pcmv mcherry cry2clust addgene (Addgene inc)

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Addgene inc 2014 pcmv mcherry cry2clust addgene
2014 Pcmv Mcherry Cry2clust Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2014+addgene/mCherry-CRY2clust+(Plasmid+%23105624)/pm41549960-464-9-11
Average 93 stars, based on 13 article reviews

2014 pcmv mcherry cry2clust addgene - by Bioz Stars, 2026-09

93/100 stars

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Flow Cytometry:

Article Title: ISL2 is a putative tumor suppressor whose epigenetic silencing reprograms the metabolism of pancreatic cancer.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Sheep anti-human ISL2 R and D Systems Cat# AF4244; RRID: AB_2296113 Mouse anti-human ISL2 Abcam Cat# ab176576 Mouse anti FLAG Sigma- Aldrich Cat# F1804; RRID: AB_262044 Flag Ab 14793S DYKDDDDK TAG (D6W5B) Cell Signaling Technology Cat# 14793; RRID: AB_2572291 Rabbit Anti-Sheep IgG H&L (HRP) (ab6747) Abcam Cat# ab6747; RRID: AB_955453 Anti-Mouse IgG (H+L), HRP Conjugate Promega Cat# W4021; RRID: AB_430834 Anti-Rabbit IgG (H+L), HRP Conjugate Promega Cat# W4011; RRID: AB_430833 Goat Anti-Mouse IgG H&L (Alexa Fluor 488) preadsorbed Antibody Abcam Cat# ab150117; RRID: AB_2688012 Goat Anti-Mouse IgG H&L (Alexa Fluor 488) antibody Abcam Cat# ab150113; RRID: AB_2576208 H3K27ac Abcam Cat# ab4729; RRID: AB_2118291 PPARg Cell Signaling Technology Cat# 2443; RRID: AB_823598 Beta Actin Sigma- Aldrich Cat# A1978; RRID: AB_476692 Bacterial and virus strains AAV-CMV-sgRNA-EF1a-Sp-Cas9 This paper NA NEB Stable competent E.coli New England Biolabs Cat# C3040H Biological samples Patient-derived xenografts (PDX) This paper NA Chemicals, peptides, and recombinant proteins 3H-Glucose Perkin Elmer Cat#NET238C001MC 14C Palmitate Perkin Elmer Cat# NEC075H050UC Optiphase Super Perkin Elmer Cat#N9309103 poly-D-lysine Millipore Cat# A003E 2-Deoxy-D-glucose Chem Impex Cat# 21916 Bam15 Cayman Cat#17811 Rotenone Millipore-Sigma Cat#R8875 Antimycin A Millipore-Sigma Cat#A8674 BIODIPY Thermo Fisher Cat#D3922 T0070907 Selleck Chemicals Cat#S2871 Metformin Selleck Chemicals Cat# S1950 Oligomycin Sigma-Aldrich Cat#75351 Phenformin Selleck Chemicals Cat# S2542 Etomoxir Cayman Chemical Cat#828934-41-4 BPTES Sigma-Aldrich Cat# SML0601 Annexin V conjugate Life Technologies Cat# A13199 Dynabeads Protein A Thermo Fisher Cat#10002D MTT Invitrogen M6494 Matrigel Thermo Fisher Cat# 356234 Caspase-3/7 dye Sartorius Cat# 4440 FxCycle Violet Stain Thermo Fisher Cat# F10347 cobalt (II) chloride Sigma- Aldrich Cat# C8661 FX11 EMD Millipore Cat# 427218 2-DG Selleck Chemicals Cat#S4701 (Continued on next page) Developmental Cell 57, 1331–1346.e1–e9, June 6, 2022 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER CAY10566 Cayman chemical Cat# #10012562 GW9662 Selleck Chemicals Cat# S2915 Critical commercial assays Click-iT Plus EdU Alexa Fluor 488 Flow Cytometry Assay Kit Thermo Fisher C10632 CellTiter Glo 2.0 Promega G924B NEBNext Ultra II DNA Library Prep Kit for Illumina New England Biolabs Cat# E7103 NEBNext Ultra Directional RNA Library Prep Kit for Illumina New England Biolabs Cat# E7420L Fast SYBR Green Master Mix applied biosystems Cat# 4385616 Deposited data RNA seq This Paper GEO: GSE180901 CHIP seq This Paper GEO: GSE180281 Experimental models: Cell lines PDX366 This Paper NA BXPC3 ATCC Cat# CRL-1687; RRID: CVCL_0186 AsPC1 ATCC Cat# CRL-1682; RRID: CVCL_0152 KP4 RCB Cat# RCB1005; RRID: CVCL_1338 H6C7 Kerafast CVCL_0P38; RRID: CVCL_0P38 hTERT FT190 Daniela Matei, Northwestern University, USA Cat# CRL-3444; RRID: CVCL_B0A3 HEK-293 ATCC Cat# CRL-1573; RRID: CVCL_0045 Experimental models: Organisms/strains Mouse: Pdx1Cre: KrasLSLG12D C57Bl/6J The Jackson Laboratory RRID: IMSR_JAX:008179 Mouse: Athymic Nude-Foxn1nu Envigo #069 Oligonucleotides ISL2_gRNA1: GCCGGCAGAGAAGCCCG GGA This Paper N/A ISL2_gRNA2: GCGACACCCGCAGGATA AAC This Paper N/A ISL2_gRNA3: GGCAGGCCGCGTGCCAC TCG This Paper N/A ISL2_qRT_F: GCAGCAACACAGTGACA AGG This Paper N/A ISL2_qRT_R: GGTACGTCTGCACCTCG ACT This Paper N/A Sg1-ISL2(promoter): GGAGATGTGCGC AAAGTGTG This Paper N/A Sg2-ISL2(promoter): GGCGCAAAGTG TGCGGCCAG This Paper N/A Sg3-ISL2(promoter): GGTTAGTTAGCA AAGAGCCG This Paper N/A Recombinant DNA P413 (LV-sgRNA(BsmBI-WTCas9) This paper N/A P82 (LV-dCas9-KRAB) This paper N/A lentiGuide-Puro Sanjana et al., 2014 Addgene #52963 pTWIST-Flag_ISL2 This paper NA (Continued on next page) e2 Developmental Cell 57, 1331–1346.e1–e9, June 6, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms FlowJo BD Biosciences V10 GraphPad Prism GraphPad Software V9 MAGeCK Li et al., 2014 https://github.com/liulab-dfci/MAGeCK HISAT2 Kim et al., 2015 http://daehwankimlab.github.io/hisat2/ StringTie Pertea et al., 2016 https://ccb.jhu.edu/software/stringtie/ DESeq2 Love et al., 2014 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html Bowtie2 Langmead and Salzberg, 2012 http://bowtie-bio.sourceforge.net/bowtie2/ index.shtml HOMER Heinz et al., 2010 http://homer.ucsd.edu/homer/ngs/ peaks.html Samtools Li et al., 2009 http://www.htslib.org/ deepTools2 Ramı́rez et al., 2016 https://deeptools.readthedocs.io/en/ develop/ ChIPseeker Yu et al., 2015 https://bioconductor.org/packages/ release/bioc/html/ChIPseeker.html

SYBR Green Assay:

RNA Sequencing:

ChIP-sequencing:

Recombinant:

Article Title: CpG island reconfiguration for the establishment and synchronization of polycomb functions upon exit from naive pluripotency.
Article Snippet: Article

Article Title: ABL kinases regulate translation in HER2+ cells through Y-box-binding protein 1 to facilitate colonization of the brain.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Anti asialo GM1 Rabbit FUJIFILM Wako Chemicals Cat#986-10001; RRID:AB_516844 Lenti-X Concentrator Takara Bio Cat #631231 DPH Sigma Cat #SML0202 GFP-Trap Agarose Chromotek Cat#gta-20 GST-ABL1 Recombinant Protein Sino Biological Cat#11199-H09B His-YB1 Recombinant Protein Novus Biologicals Cat#NBP2-30101 Staurosporine Sigma Cat#S6942 Anti-FLAG M2 Affinity Gel Sigma Cat#A2220 PureLink RNase A Thermo Fisher Cat#12091021 Critical commercial assays Pierce Magnetic RNA-Protein Pull-Down Kit Thermo Fisher Cat# 20164 Pierce RNA 30 End Desthiobiotinylation Kit Thermo Fisher Cat# 20163 Pierce Silver Stain for Mass Spectrometry Thermo Fisher Cat# 24600 MagnaRIP RNA-Binding Protein Immunoprecipitation Kit Sigma Cat#17-700 Cell Titer Glo Promega Cat# G7571 Hoescht 33342 Solution Thermo Fisher Cat# 62249 Caspase-Glo 3/7 Assay Promega Cat#G8091 Deposited data HER2 mRNA Binding Protein Pulldown Mass Spectrometry Data This paper MassIVE: MSV000087674 Original, Unprocessed Data This paper Mendeley Data: https://doi.org/10.17632/ nzsk26yf5x.1 YB-1 HITS-CLIP dataset for long RNA species Goodarzi et al., 2015 GEO: GSE63604 Metastatic Breast Cancer Project dataset Metastatic Breast Cancer Project cBioPortal INSERM Metastatic Breast Cancer dataset Lefebvre et al., 2016 EGA: EGAS00001001695 TCGA Breast Invasive Carcinoma dataset TCGA, Firehose Legacy cBioPortal Sanger Breast Invasive Carcinoma dataset Stephens et al., 2012 EGA: EGAD00001000133 Broad Breast Invasive Carcinoma dataset Banerji et al., 2012 dbGaP: phs000369.v1.p1 British Columbia Breast Invasive Carcinoma dataset Shah et al., 2012 EGA: EGAS00001000132 SMC Breast Cancer dataset Kan et al., 2018 GEO: GSE113184 MSKCC Breast Cancer dataset Nixon et al., 2019 cBioPortal METABRIC Breast Cancer dataset Curtis et al., 2012; Pereira et al., 2016 EGA: EGAS00001001753; EGAS00000000083 MSK Breast Cancer dataset Razavi et al., 2018 dbGaP: phs001674.v1.p1 Experimental models: Cell lines HCC1954-LCC1 Malladi et al., 2016 N/A ErbB2-BrM2 Valiente et al., 2014 N/A SUM190-BrM Gril et al., 2018 N/A NCI-H1781 ATCC Cat# CRL-5894; RRID: CVCL_1494 HEK-293T ATCC Cat# CRL-3216; RRID: CVCL_0063 Experimental models: Organisms/strains Outbred athymic nu/nu mice Jackson Laboratory Cat#007850; RRID: IMSR_JAX:007850 FVB/NJ Jackson Laboratory Cat#001800; RRID: IMSR_JAX:001800 Oligonucleotides See Table S4 for a list of all primers and oligonucleotides (Continued on next page) e2 Cell Reports 40, 111268, August 30, 2022 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pLKO-puro Non-Target shRNA Control Sigma Mission TRC1 SHC016-1EA pLKO-puro shAGO1 Sigma Mission TRC1 TRCN0000007861 pLKO-puro shAGO2 Sigma Mission TRC1 TRCN0000007865 pLKO-puro shAGO3 Sigma Mission TRC1 TRCN0000007870 pLKO-puro shAGO4 Sigma Mission TRC1 TRCN0000007875 pLKO-puro shAGO4 Sigma Mission TRC1 TRCN0000007874 pLKO-puro shYB1 #1 Sigma Mission TRC1 TRCN0000007951 PLKO-puro shYB1 #2 This paper N/A pLKO-puro shYB1 #3 Sigma Mission TRC1 TRCN0000007948 pLKO-puro shSSB Sigma Mission TRC1 TRCN0000062197 pLKO-puro shHER2 Sigma Mission TRC1 TRCN0000010341 pLKO-puro shHER2 Sigma Mission TRC1 TRCN0000010342 pLKO-puro shAXL #1 Sigma Mission TRC1 TRCN0000000572 pLKO-puro shAXL #2 Sigma Mission TRC1 TRCN0000000573 pLKO-puro shL1CAM #1 Sigma Mission TRC1 TRCN0000063913 pLKO-puro shL1CAM #2 Sigma Mission TRC1 TRCN0000063914 Scramble control shRNA Gu et al., 2016 N/A ABL1 shRNA Gu et al., 2016 N/A ABL2 shRNA Gu et al., 2016 N/A pBABEpuro-ERBB2 Greulich et al., 2012 Addgene #40978; RRID: Addgene_40978 lentiCas9-Blast Sanjana et al., 2014 Addgene #52962; RRID: Addgene_52962 LentiGuide-Hygro http://n2t.net/addgene:139462 Addgene #139462; RRID: Addgene_139462 LentiGuide-Neo Doyle et al., 2018 Addgene #139449; RRID: Addgene_139449 LentiGuide-Puro Sanjana et al., 2014 Addgene #52963; RRID: Addgene_52963 LentiGuide-sgRNA human ABL1 #1-Neo This paper N/A LentiGuide-sgRNA human ABL1 #2-Neo This paper N/A LentiGuide-sgRNA human ABL2 #1-Hygro This paper N/A LentiGuide-sgRNA human ABL2 #2-Hygro This paper N/A LentiGuide-sgRNA mouse ABL1 #1-Hygro This paper N/A LentiGuide-sgRNA mouse ABL2 #1-Puro This paper N/A pLVX-Tet-On Advanced Clontech Ref# 632162 TetO-FUW-pgk-puro Chowdhury et al., 2016 Addgene #85747; RRID: Addgene_85747 TetO-FUW-HER2-pgk-puro This paper N/A pcDNA-ABL1PP Plattner et al., 2004 N/A pcDNA-ABL2PP Plattner et al., 2004 N/A mEGFP-c1 http://n2t.net/addgene:54759 Addgene #54759; RRID: Addgene_54759 mEGFP-YB1-c1 This paper N/A pcDNA-GFP-ABL1 Burton et al., 2003 N/A GFP-ABL1PP This paper N/A mEGFP-YB1 A/P Domain-c1 This paper N/A mEGFP-YB1 CSD-c1 This paper N/A (Continued on next page) Cell Reports 40, 111268, August 30, 2022 e3 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER mEGFP-YB1 CSD Y72F-c1 This paper N/A mEGFP-YB1 CSD Y99F-c1 This paper N/A mEGFP-YB1 C-Term Domain-c1 This paper N/A 3xFLAG-FUS-WT Yamazaki et al., 2012 Addgene #44985; RRID: Addgene_44985 3xFLAG-YB-1 This paper N/A N174-MCS http://n2t.net/addgene:81061 Addgene #81061; RRID: Addgene_81061 N174-eGFP-YB-1-MCS This paper N/A perbB2-ECFP Offterdinger and Bastiaens, 2008 Addgene #40268; RRID: Addgene_40268 pDESTmycYBX1 Landthaler et al., 2008 Addgene #19878; RRID: Addgene_19878 Software and algorithms Prism 8 Graphpad http://graphpad.com/scientific-software/prism ImageJ Schneider et al., 2012 http://imagej.nih.gov Living Image Perkin Elmer http://perkinelmer.com CLIP Tool Kit Shah et al., 2017 https://zhanglab.c2b2.columbia.edu/ index.php/CTK_Documentation Article ll OPEN ACCESS

Article Title: Make war not love: The neural substrate underlying a state-dependent switch in female social behavior.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains AAV2-EF1a-DIO-hChR2(H134R)-EYFP UNC vector core N/A AAV5-EF1a-DIO-eNpHR3-EYFP UNC vector core; Gradinaru et al., 2010 Addgene# 26966 AAVDJ-hSyn-Coff/Fon-hChR2(H134R)-EYFP UNC vector core; Fenno et al., 2014 Addgene# 55648 AAVDJ-Syn-Coff/Fon-EYFP UNC vector core; Fenno et al., 2014 Addgene# 55652 AAV1-Syn-Flex-GCaMP6m Addgene; Chen, 2013 Addgene# 100838 AAV8-nEF-Coff/Fon-eNpHR3.3-EYFP Addgene; Fenno et al., 2020 Addgene# 137154 AAVDJ-EF1a-Coff/Fon-GCaMP6m Addgene; Fenno et al., 2020 Addgene# 137121 Chemicals, peptides, and recombinant proteins Papain Sigma Cat# P3125-250MG Egg White/BSA ovomucoid inhibitor Worthington Cat# OI-BSA DNase I Thermo scientific Cat# 90083 DNase/RNase-Free Distilled Water Thermo scientific Cat# 10977-015 DAPI Sigma Cat# D9542-10MG BSA Thermo scientific Cat# AM2616 Actinomycin D Sigma Cat# A1410-2MG Kynurenic acid sodium salt L-Cysteine Abcam Cat# ab120256 L-Cysteine Sigma Cat# W326305-100G D-(+)-Trehalose dihydrate Sigma Cat# 90210-50G Ethylenediaminetetraacetic acid Sigma Cat# 03690-100ML MgSO4 solution Sigma Cat# 83266-100ML-F CaCl2 solution Sigma Cat# 21115-100ML Sodium nitrite Sigma Cat# 237213-100G Heparin sodium salt Sigma Cat# H4784-250MG Paraformaldehyde EMS Cat# 15714-S Sucrose, RNase & DNase Free Amresco Cat# 0335-2.5KG Critical commercial assays Chromium Next GEM Single Cell 3’ GEM, Library & Gel Bead Kit v3.1 10x Genomics PN-1000121 Chromium Next GEM Chip G Single Cell Kit 10x Genomics PN-1000120 Chromium i7 Multiplex Kit 10x Genomics PN-120262 Experimental models: Organisms/strains Mouse: Esr1-flpo Karigo et al., 2020 JAX No. 036028 Mouse: Esr1-cre Lee et al., 2014 JAX No. 017911 Mouse: Npy2r-cre Chang et al., 2015 JAX No. 029285 Mouse: C57BL/6N Charles River N/A Mouse: BALB/c Charles River N/A Software and algorithms CellRanger v3 10x Genomics http://www.10xgenomics.com/ Python v3 Python Software Foundation http://www.python.org/ R v3.6.2 R Foundation R Foundation https://www.r-project.org/ Seurat v3 Stuart et al., 2019 https://satijalab.org/seurat/ 10x analysis code (R and python) This paper N/A Photometry analysis code (python) This paper N/A (Continued on next page) Neuron 110, 841–856.e1–e6, March 2, 2022 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Prism 6 GraphPad Software https://www.graphpad.com/ ImageJ NIH https://imagej.nih.gov/ij Deposited data 10x Seq data This paper GEO: GSE193921; Mendeley Data: https://dx.doi.org/10.17632/w25zm9cn6x.1

other:

Article Title: BAG6 prevents the aggregation of neurodegeneration-associated fragments of TDP43.
Article Snippet: Novagen (EMD Millipore) pX330-U6-Chimeric_BB-CBh-hSpCas9: AmpR; Mammalian expression plasmid encoding a human codon-optimized SpCas9 and a chimeric guide RNA Cong et al., 2013 Addgene Cat#42230 pCB264: AmpR; NeoR; pcDNA3.0-based plasmid encoding fDHFR-UbK48R-Val-Ab(11-42)13myc under the control of T7 or CMV promoter Brower et al., 2013 N/A pCB323: AmpR; NeoR; pcDNA3.0-based plasmid encoding fDHFR-UbK48R-Met208-TDP43f under the control of T7 or CMV promoter Brower et al., 2013 N/A pCB328: AmpR; NeoR; pcDNA3.0-based plasmid encoding fDHFR-UbK48R-Val219-TDP43f under the control of T7 or CMV promoter Brower et al., 2013 N/A pCB332: AmpR; NeoR; pcDNA3.0-based plasmid encoding fDHFR-UbK48R-Val247-TDP43f under the control of T7 or CMV promoter Brower et al., 2013 N/A pCB385: AmpR; pcDNA3.0-based plasmid encoding fDHFR-UbK48R-Val-Tau3-124 under the control of T7 or CMV promoter Brower et al., 2013 N/A pCB396: AmpR; NeoR; pcDNA3.0-based plasmid encoding fDHFR-UbK48R-Val-CTFbf under the control of T7 or CMV promoter Brower et al., 2013 N/A pCB399: AmpR; pGL3-Control-based plasmid encoding mCherry-UbK48R-Val219-TDP43f under the control of SV40 promoter Kasu. et al., 2018 N/A pYK27: AmpR; NeoR; pcDNA3.0-based plasmid encoding fDHFR-UbK48R under the control of T7 or CMV promoter Kasu. et al., 2018 N/A pRK5-FLAG-Bag6: AmpR; NeoR; pRK5-based plasmid encoding fBAG6 under the control of CMV promoter Liu et al., 2014 Addgene Cat#61836 pRK5-FLAG-Bag6DUBL: AmpR; NeoR; pRK5-based plasmid encoding fBAG6DUBL under the control of CMV promoter Liu et al., 2014 Addgene Cat#61837 pAAV-Ef1a-Cre: AmpR; AAV-based plasmid encoding Cre recombinase under the control of EF1a promoter Fenno et al., 2014 Addgene Cat#55636 p571: KanR; NeoR, pCMV6-Entry-based plasmid encoding myc-fTRC35 under the control of CMV promoter Origene Cat#RC200220 p572: KanR; NeoR, pCMV6-Entry-based plasmid encoding myc-fUBL4A under the control of CMV promoter Origene Cat#RC208121 p573: KanR; NeoR, pCMV6-Entry-based plasmid encoding myc-fRNF126 under the control of CMV promoter Origene Cat#RC200161 GST-RNF126: AmpR; pGEX-4T3 based plasmid for bacterial expression of GSTRNF126 Smith et al., 2013 Addgene Cat#138643 GST-RNF126C231A: AmpR; pGEX-4T3 based plasmid for bacterial expression of GSTRNF126C231A Smith et al., 2013 Addgene Cat#138644 pYK33: AmpR; pET16b-based plasmid for bacterial expression of Met219-TDP43f-6XHis This study N/A (Continued on next page) iScience 25, 104273, May 20, 2022 17

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2014 pcmv mcherry cry2clust addgene (Addgene inc)

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